@article{EunheeKim2023,
title = {A Neurospheroid-Based Microrobot for Targeted Neural Connections in a Hippocampal Slice},
author = {Eunhee Kim and Sungwoong Jeon and Yoon-Sil Yang and Chaewon Jin and Jin-young Kim and Yong- Seok Oh and Jong-Cheol Rah and and Hongsoo Choi},
url = {https://onlinelibrary.wiley.com/doi/10.1002/adma.202208747?af=R},
doi = {https://doi.org/10.1002/adma.202208747},
year = {2023},
date = {2023-01-14},
journal = {Advanced Materials},
abstract = {Functional restoration by the re-establishment of cellular or neural connections remains a major challenge in targeted cell therapy and regenerative medicine. Recent advances in magnetically powered microrobots have shown potential for use in controlled and targeted cell therapy. In this study, a magnetic neurospheroid (Mag-Neurobot) that could form both structural and functional connections with an organotypic hippocampal slice (OHS) was assessed using an ex vivo model as a bridge toward in vivo application. The Mag-Neurobot consists of hippocampal neurons and superparamagnetic nanoparticles (SPIONs); it is precisely and skillfully manipulated by an external magnetic field. Furthermore, the results of patch-clamp recordings of hippocampal neurons indicated that neither the neuronal excitabilities nor the synaptic functions of SPION-loaded cells were significantly affected. Analysis of neural activity propagation using high-density multi-electrode arrays showed that the delivered Mag-Neurobot was functionally connected with the OHS. The applications of this study include functional verification for targeted cell delivery through the characterization of novel synaptic connections and the functionalities of transported and transplanted cells. The success of the Mag-Neurobot opens up new avenues of research and application; it offers a test platform for functional neural connections and neural regenerative processes through cell transplantation.},
keywords = {MaxOne, microrobot, Organoids, Slices},
pubstate = {published},
tppubtype = {article}
}

@article{Kim2020,
title = {A magnetically actuated microrobot for targeted neural cell delivery and selective connection of neural networks},
author = {Eunhee Kim and Sungwoong Jeon and Hyun-Kyu An and Mehrnoosh Kianpour, Seong-Woon Yu and Jin-young Kim and Jong-Cheol Rah and Hongsoo Choi},
url = {https://advances.sciencemag.org/content/6/39/eabb5696},
doi = {10.1126/sciadv.abb5696},
year = {2020},
date = {2020-09-25},
journal = {Science Advances},
volume = {6},
number = {39},
abstract = {There has been a great deal of interest in the development of technologies for actively manipulating neural networks in vitro, providing natural but simplified environments in a highly reproducible manner in which to study brain function and related diseases. Platforms for these in vitro neural networks require precise and selective neural connections at the target location, with minimal external influences, and measurement of neural activity to determine how neurons communicate. Here, we report a neuron-loaded microrobot for selective connection of neural networks via precise delivery to a gap between two neural clusters by an external magnetic field. In addition, the extracellular action potential was propagated from one cluster to the other through the neurons on the microrobot. The proposed technique shows the potential for use in experiments to understand how neurons communicate in the neural network by actively connecting neural clusters.},
keywords = {microrobot, Neuronal Networks},
pubstate = {published},
tppubtype = {article}
}