MaxWell Biosystems at ISSCR 2022
Discover all our events and activities that will be taking place during the ISSCR Annual Meeting 2022 in San Francisco from Tuesday June 15 – Saturday June 18!
Meet the Team at ISSCR:
Our MaxWell representatives will be at ISSCR and would love to meet you!
Dr. Urs Frey
Dr. Marie Obien
Martina de Gennaro
Field Application Scientist
Dr. Zhuoliang (Ed) Li
Field Application Scientist
Dr. Diana Freire
Scientific Marketing Specialist
Associate Application Scientist
Visit booth #702 to learn more about our HD-MEA systems MaxOne and MaxTwo.
June 17 11:30 – 12:30 (PDT)
Title | Next-generation in-vitro assays: Characterizing the activity of human iPSC-derived neurons in 2D and 3D cultures at high resolution
Abstract | Both 2D and 3D brain models derived from human induced pluripotent stems cells (hiPSCs) are emerging as promising tools for investigating brain development and disease progression, as well as to test drug toxicity and efficacy in-vitro. In order to adopt hiPSC-derived 2D and 3D neuronal networks for rapid and cost-effective phenotype characterization and drug screening, it is necessary to assess their cell type composition, gene expression patterns, and physiological function.
In this innovation showcase, our invited speakers will showcase studies where MaxWell Biosystems’ advanced high-density microelectrode arrays (HD-MEAs) as the core of easy-to-use platforms, MaxOne (single-well) and MaxTwo (multi-well), allowed to capture neuronal activity across multiple scales, from sub-cellular to single cells, up to full networks and facilitated the characterization of the neuronal activity of hiPSC-derived neurons. During this session speakers will introduce how brain development disorders are modeled in 2D and 3D in-vitro. Overall, the presentations will provide an overview on how HD-MEA technology can efficiently advance research in 2D and 3D hiPSC-derived brain models and accelerate drug discovery for neurodegenerative diseases.
Onsite Speakers | Schedule:
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|June 17 | 11:30 - 11:32||Dr. Marie Obien|
CCO, MaxWell Biosystems, Host of the session
|11:32 - 11:40||Dr. Urs Frey|
CEO, MaxWell Biosystems
MaxWell Biosystems Welcome Address
|11:40 - 12:00||Dr. Bruna Paulsen|
Arlotta Lab, Harvard University, USA
Dr. Silvia Velasco
Murdoch Children's Research Institute, Australia
Talk | Human brain organoids reveal asynchronous development of cortical neuron classes as a shared feature of autism risk genes
Genetic risk for autism spectrum disorder is associated with mutations in hundreds of genes; however, neurodevelopmental abnormalities resulting from these mutations remain unclear. Here, we will describe how human brain organoids have been recently used to uncover shared cell type-specific neurodevelopmental abnormalities among three autism risk genes. Our data show a convergent phenotype of asynchronous development of two main cortical neuronal lineages and suggests that a shared clinical pathology may derive from higher-order processes of neuronal differentiation and circuit wiring.
|12:00 - 12:20|| Dr. Marián Hruška-Plocháň|
Polymenidou Lab, University of Zurich, Switzerland
Talk | Human neural networks with sparse TDP-43 pathology reveal NPTX2 misregulation in ALS/FTLD
Human cellular models of neurodegeneration require reproducibility and longevity. To explore the TDP-43 pathologies, we generated iPSC-derived, colony morphology neural stem cells (iCoMoNSCs) that differentiated into a self-organized multicellular system, which matured into long-lived functional neural networks. Overexpression of TDP-43 in neurons led to progressive fragmentation and aggregation, resulting in loss of function and neurotoxicity. The strongest RNA target revealed by scRNA-seq encoded for NPTX2, which was misaccumulated in ALS and FTLD patient neurons with TDP-43 pathology.
|12:20 - 12:30||Q&A Session|
Online Speakers | Schedule:
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|June 17 | 11:30 - 11:45||Dr. David Jäckel|
Senior Product Manager, MaxWell Biosystems, Switzerland
Talk | AxonTracking Assay: high-resolution and high-throughput mapping of propagating action potentials
Axonal dysfunction plays a central role in deliberating pathologies. Therefore, access to axonal physiology is crucial for studying information processing within neuronal networks and accelerating drug development for neurological disorders. In this talk, we present the MaxLab Live AxonTracking Assay, a novel tool to automatically detect and functionally characterize axonal neuronal signals in neuronal networks grown on high-resolution microelectrode arrays. We used the AxonTracking Assay to reliably measure from Human iPSC-derived neurons over multiple weeks and to extract key metrics such as conduction velocity, axonal length, and axonal latency. We found a significantly increased axonal velocity in human motor neurons as compared to dopaminergic and glutamatergic neurons.
|11:45 - 12:05||Dr. David Pamies|
Zurich Fontanellaz Lab, University of Lausanne, Switzerland
Talk | Evaluation of multi-well microelectrode arrays (MEA) for neurotoxicity screening in a 3D human brain iPSC-derived model using a chemical training set
The use of Multielectrode arrays (MEAs) have been proposed as an in vitro neurotoxicity screening method and a recent study demonstrated high reproducibility and reliability of MEA measurements across five laboratories. However, most of the work has been done in 2D cortical rat cultures. The development of new primary human cell culture technologies such as 3D culture in combination with iPSC derived models promise to generate more relevant human physiological systems. In order to internal validate the model, we have performed a set of chemical experiments by using the MaxTwo platform using various concentrations and different time points.
|12:05 - 12:30||Dr. Kenta Shimba|
Jimbo Lab, University of Tokyo, Japan
Talk | High resolution spatiotemporal evaluation of rat sensory axons
Axons are considered to play an important role in the computational functions in central and peripheral nervous system. Although electrophysiological methods have been used to characterize conduction properties, spatial and temporal resolution has been limited. We have been studying relationships between axonal structure and conduction properties using MaxOne system. In my presentation, I will talk about recent advances in our study on rat sensory axons.
|Date and Time||Thursday June 16 15:00-16:00 | Onsite|
|Poster Title||ELECTROPHYSIOLOGICAL PHENOTYPE CHARACTERIZATION OF HEALTHY AND DISEASED HUMAN IPSC-DERIVED MOTOR NEURONS BY MEANS OF HIGH-DENSITY MICROELECTRODE ARRAYS|
|Presenting||Dr. Marie Obien|
|Date and Time||Thursday June 16 16:00-17:00 | Onsite|
|Poster Title||ELECTROPHYSIOLOGICAL CHARACTERIZATION OF NEURONS MODELING NEUROLOGICAL DISEASES USING HIGH-DENSITY MICROELECTRODE ARRAYS|
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