MaxLab Live Software Modules

MaxOne Basic

The Basic Package allows access to 256 recording electrodes arranged in four different spatial configurations. MaxOne Basic combines the capability of 4 traditional microelectrode arrays (MEAs) in one device.

Medium-density

  • 2×2 sq-mm total area coverage
  • 122.5 μm electrode spacing
  • Best used for population recordings covering a large area

High-density

  • 0.84×0.84 sq-mm total area coverage
  • 52.5 μm electrode spacing
  • Best used for axonal action potential tracking; facilitates spike sorting

Ultra high-density

  • 0.28×0.28 sq-mm total area coverage
  • 17.5 μm electrode spacing
  • Best used for sub-cellular recording of local networks; optimal spike sorting results

Sparse with 4-el groups

  • 1.5×1.5 sq-mm total area coverage
  • 17.5 μm spacing within 4-electrode groups
  • 210 μm electrode group spacing
  • Combines large area coverage and high-density configuration to facilitate spike sorting in population recordings

Additional Software Modules

Max Channels Module

MaxOne allows increasing the number of simultaneously recorded electrodes up to 4 times (1024 recording channels in total) through the Max Channels Module. Increasing the number of electrodes gives access to larger area coverage, both in sparse and high-density configurations.

Routing Flexibility Module

MaxOne has 26,400 electrodes in total with 17.5 μm spacing. The full sensor area is 3.85×2.10 sq-mm. With the Routing Flexibility Module, the the user gets unlimited number of electrode configurations that can be used per experiment.This module is required to enable Whole Sample Electrical ImagingSmart Population Recording, and Axon Tracking recording modes (see MaxLab Live Recording Modes below for more information).

Stimulation Module

The Stimulation Module upgrades MaxOne Basic to have 32 stimulation channels. Each stimulation channel can deliver up to ± 1.6 V voltage or ± 1.5 mA current amplitude. The amplitude resolution is 2 nA and time resolution is 2 μs. MaxLab Live allows flexible pattern generation, such as monophasic, biphasic, triphasic pulses, ramp waveform, and other custom pulse shapes. MaxLab Live can be used to visualize elicited single cell activity by electrical stimulation.

* MaxOne Full Package includes all of the three additional modules.

MaxLab Live Recording Modes

Whole Sample Electrical Imaging

The Routing Flexibility Module allows the use of multiple high-density configurations to scan through all the 26,400 electrodes. The user can also choose a custom area to scan, instead of the full array.

The images below show the overlap between the optical image (fluorescence microscopy) and the electrical image (MaxOne with routing flexibility module) overlap in primary cortical cell culture. The electrical image provides the location of the cells, as well as information on the activity of the cells, such as spike rate and amplitude.

Together with 1024 Recording Channels Module, a full activity scan can be performed within a few minutes for very active cell culture and tissue samples.

Smart Population Recording

After performing whole sample electrical imaging, MaxLab Live Data Analysis Toolbox enables localization of defined cells for long term recording and further analyses. The user can choose among different combinations of parameters for selecting a defined set of cells to be recorded, e.g., spike rate, spike amplitude, spike sortability.

Upon determining the location of the cells, the Routing Flexibility Module can be used to automatically create electrode configurations to be used for long term recording. The image below shows a raster plot with 1024 pre-selected channels recorded for 100 seconds. A zoom in to one population burst is also shown.

Axon Tracking

MaxOne equips the user with single-neuron tracking at sub-cellular resolution. MaxOne captures the initiation of the action potential at the AIS and tracks the propagation of the action potential along the full axonal arbor. With the Routing Flexibility Module, the user is able to scan the full array while tracking single neurons of interest. By averaging the spikes obtained from either spontaneous activity or stimulation-evoked activity, the full axonal arbor can be revealed.

After the initial tracking process, the MaxLab Live Data Analysis Toolbox can be used to identify the axonal arbors. New electrode configurations can be used using the Routing Flexibility Module to track the cell axonal arborization for long-term recordings across multiple days and months. This method is best used to investigate the changes in axonal conduction velocity during development and upon application of compounds.

The images below show a full neuronal axon arbor tracked using MaxOne.

Custom Recording Protocols

Depending on the needs of the application, the user can design recording protocols tailored for the experiment and data of interest. MxW Support Team is happy to discuss your specific application. Our support team has both technical and neuroscience expertise to help you design your experiments according to your research question. Contact us directly by email or through the online form.

 

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