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Impaired Activity in Motor Neurons modeling ALS with Daniel Sommer
October 4, 2022
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Dr. Marie Obien Daniel Sommer Abstract Background: Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease, affecting both upper and lower motor neurons (MN) and that leads to death typically within 3-5 years following diagnosis. Even though previous studies revealed that alterations in synapses and neuronal activity are part of the underlying pathomechanisms in both in vitro and in vivo models, their specific contribution to neurodegenerative processes is still under debate. Specifically, the influence of neuronal hyper- or hypoactivity on cellular disease progression is highly controversial since both phenotypes have been described and found to be harmful in ALS MN. Methods: We employed high density multielectrode array (HD-MEA) techniques to longitudinally monitor the electrophysiological properties of hiPSC-derived C9orf72-mutant and healthy MN. To gain further insight into molecular causes triggering activity alterations in mutant motor neurons, we combined this data with corresponding transcriptome analyses. Finally, we sought to rescue the observed phenotype by administration of the SK channel blocker Apamin. Results: In our study, we found an early hyperactivity of ALSC9orf72 MN, which drastically decreased upon neuronal aging and was no longer evident when neurodegeneration started to occur. In accordance with previous publications describing synaptic loss in ALS MN, we could furthermore observe a generally reduced network synchroneity in ALSC9orf72 MN cultures. Consistent with our HD-MEA findings, we observed an up-regulation of synaptic transcripts in ALSC9orf72 MN at the earlier time point, which was followed by a significant reduction over time. By administration of the SK channel inhibitor Apamin, which has previously been shown to be neuroprotective in ALS MN, we were able to achieve beneficial effects on an electrophysiological as well as transcriptional level. Conclusion: Altogether, this study suggests phenomena of synaptic maturation as possible explanation for contradicting evidence on electrophysiological alterations in ALSC9orf72 MN, provides an insight into the longitudinal development of their neuronal activity and links these functional changes to aging-dependent transcriptional programs.More details
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Speaker
MaxWell Biosystems
Switzerland
AG Catanese: Cell Biology of Neurodegenerative Diseases
Ulm University, Germany
What’s your cell story?
Characterizing the activity of human iPSC derived-neurons in 2D and 3D cultures at high resolution
November 11, 2021
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Dr. Marie Obien Dr. Silvia Ronchi Giulio Zorzi Abstract Alongside our participation at the SfN Annual Meeting 2021, we will host two sessions presenting you replays of a Case Study presentation based on a paper published at Advanced Biology and our MaxTwo showcase. Both will be followed by a Live Q&A session. During these sessions, we will introduce our technology, products and applications and allow all attendees to ask questions to our team during the live Q&A session. These sessions will be hosted by Dr. Marie Obien who will briefly introduce the company, technology, products and applications. The case study is presented by Dr. Silvia Ronchi, which is entitled, “Electrophysiological phenotype characterization of human iPSC derived-neuronal cell lines by means of high-density microelectrode arrays”, highlighting how the neuronal activity in 2D samples can be easily captured, label-free, at single-cell resolution by using MaxWell Biosystems’ high-density microelectrode array (HD-MEA) platforms. Giulio Zorzi showcases MaxTwo, a powerful system to characterize the function of human iPSC-derived neurons that can help you advance your research in different applications. The presentations will be followed by a live Q&A session. Overall, the presentations will provide an overview on how HD-MEA technology can efficiently advance research in 2D and 3Dhuman derived from induced pluripotent stems cells (hiPSCs) brain models, as promising tools for investigating development, disease progression, and to test drug toxicity/efficacy in-vitro, and accelerate drug development for neurodegenerative diseases.More details
Host
CCO at MaxWell BiosystemsSpeaker
Scientific Application Specialist at MaxWell Biosystems
Product Manager & Application Engineer at MaxWell Biosystems
Presenting MaxTwo: A powerful system to characterize the function of human iPSC-derived neurons
September 29, 2021 | 10am / 5pm (CET), 1am / 8am (PDT), 4am / 11am (EDT), 5pm / 12am (JST)
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Dr. Marie Obien Giulio Zorzi Abstract In our live broadcast from our laboratory here in Zurich, Switzerland, we will:More details
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Speaker
VP Marketing and Sales at MaxWell Biosystems
Product Manager & Application Engineer at MaxWell Biosystems
Phenotyping of neurodevelopmental and psychiatric disorders with human iPSC-derived dopaminergic neurons
July 8, 2021 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. Maria Sundberg Hannah Pinson Abstract In recent years, the genetic causes of autism and schizophrenia have been studied intensively. In addition to monogenic deficits, deletions or duplications of specific chromosomal loci have also been associated with neurodevelopmental and psychiatric disorders. One of these regions is 16p11.2, which contains 29 protein coding genes, most of which are also expressed in the brain. Clinical studies have shown that deletion of 16p11.2 leads to severe developmental deficits, intellectual disability, and autism. On the other hand, patients with duplication of 16p11.2 locus have an increased risk of developing schizophrenia, bipolar disorder, depression and autism. Deficits in the dopamine signaling can cause behavioral problems and deficits in social interactions in the patients with autism and schizophrenia. In this webinar, our speakers will:More details
Host
VP Marketing and Sales at MaxWell BiosystemsSpeaker
Research Fellow, Group of Prof. Sahin, Boston Children’s Hospital
PhD Candidate, Group of Prof. Ginis, Vrije Universiteit Brussel & Visitor in Prof. Tegmark group, MIT
SpikeInterface, a Unified Framework for Spike Sorting
March 25, 2021 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. Szilard Sajgo Dr. Alessio Buccino Abstract Understanding how assemblies of neurons encode information requires recording of large populations of cells. In recent years, high-density multi-electrode arrays (HD-MEAs) have been developed to record simultaneously from thousands of electrodes. Each electrode records from multiple surrounding neurons at the same time. In order to assign electrical signals recorded by HD-MEAs to individual neurons, a critical step called spike sorting needs to be performed. During this step, the extracellular action potentials originating from hundreds to thousands of neurons need to be disentangled from the background noise and from each other. We had an introduction to spike sorting in a webinar earlier this year (link to the replay) and are happy to host this new webinar as a follow-up. Dr. Szilárd Sajgó, R&D scientist at MaxWell Biosystems, will: This is followed by Dr. Alessio Buccino, ETH Postdoctoral Fellow in the group of Prof. Hierlemann, who will:More details
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VP Marketing and Sales at MaxWell BiosystemsSpeaker
Speaker
Application Specialist at MaxWell Biosystems
Postdoctoral Fellow at ETH Zürich
Morphological, functional & transcriptomic correlation of retinal organoids to the human retina
February 18, 2021 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. Szilard Sajgo Martina De Gennaro Abstract Genetic disorders of the human retina cause visual impairment to millions of people worldwide. The retina is a well characterized tissue at the back of the eye, and is a potential target for visual restoration therapies. Light-sensitive human retinal organoids recapitulate cell-types, circuitry and transcriptomic profiles of the human retina, offering a relevant tool for translational studies. In this webinar, Dr. Szilárd Sajgó, our R&D scientist and an expert in retina research will: This is followed by Ms. Martina De Gennaro, Doctoral Researcher at Institute of Molecular and Clinical Ophtalmology Basel (IOB) and co-author of this recent Cell paper, who will:More details
Host
VP Marketing and Sales at MaxWell BiosystemsSpeaker
Speaker
Application Specialist at MaxWell Biosystems
Doctoral Researcher at Institute of Molecular and Clinical Ophtalmology Basel (IOB)
Fast and Accurate Spike Sorting for Thousands of Channels
January 14, 2021 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. Szilard Sajgo Dr. Pierre Yger Abstract Understanding how assemblies of neurons encode information requires recording of large populations of cells. In recent years, high-density multi-electrode arrays (HD-MEAs) and silicon probes have been developed to record simultaneously from thousands of electrodes. Each electrode records from multiple surrounding neurons at the same time. In order to assign electrical signals recorded by HD-MEAs to individual neurons, a critical step called spike sorting needs to be performed . During this step, the extracellular action potentials originating from hundreds to thousands of neurons need to be disentangled from the background noise and from each other. In this webinar, our speakers will:More details
Host
VP Marketing and Sales at MaxWell BiosystemsSpeaker
Speaker
Application Specialist at MaxWell Biosystems
Researcher at Institut de la Vision
Co-hosted with FUJIFILM Cellular Dynamics:
Electrophysiological Phenotype Characterization of Human iPSC-Derived Neuronal Cell Lines by Means of High-Density Microelectrode Arrays
October 15, 2020 | 5pm (CET), 8am (PST), 11am (EDT)
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Marie Obien, Ph.D. Simon Hilcove, Ph.D. Silvia Ronchi Recent advances in the field of cellular reprogramming have opened a route to study the fundamental mechanisms underlying common neurological disorders. High-density microelectrode-arrays (HD-MEAs) provide unprecedented means to study neuronal physiology at different scales, ranging from network through single-neuron to subcellular features. In this webinar, we will:More details
Host
Host
VP Marketing and Sales at MaxWell Biosystems
Assoc. Director Product Development at FUJIFILM Cellular DynamicsSpeaker
Doctorate Student at ETH ZürichAbstract:
Label-free functional characterization of 3D organoids at single-cell resolution
September 10, 2020 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. Szilard Sajgo Abstract Human organoids that originate from human induced pluripotent stem cells (h-iPSCs) are emerging as promising tools for investigating development and disease progression. In order to adopt human organoids for rapid and cost-effective drug screenings, it is necessary to assess their cell type composition, gene expression patterns and physiological function. The electrical activity of brain, retina or muscle organoids can now be easily captured, label-free, at single-cell resolution by using MaxWell Biosystems’ high-density microelectrode array (HD-MEA) technology. In this webinar, the speakers will:More details
Host
Speaker
VP Marketing and Sales at MaxWell Biosystems
Application Specialist at MaxWell Biosystems
Featuring MaxLab Live: All-in-One Software for HD-MEA
July 21, 2020 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. David Jäckel Abstract The high-density microelectrode arrays (HD-MEAs) high-content electrophysiology platforms MaxOne and MaxTwo allow label-free recording and stimulation of every active cell on a dish at unprecedented spatio-temporal resolution. A powerful and easy-to-use software interface is key to reveal the full potential of this technology. MaxLab Live is an all-in-one software for live visualization, recording, and analysis of extracellular HD-MEA signals from different biological preparations. In this webinar, the speakers will:More details
Host
Speaker
VP Marketing and Sales at MaxWell Biosystems
Senior Product Manager at MaxWell Biosystems
Assessing retinal function in health and disease at single-cell resolution
June 11, 2020 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. Szilard Sajgo Abstract Genetic diseases of the human retina cause visual impairment to millions of people worldwide. The retina is a well characterized tissue at the back of the eye, and is a potential target for visual restoration therapies. The efficacy of these therapies can now be easily assessed using high-density microelectrode arrays (HD-MEA). In addition, HD-MEA technology can also be used to assess disease phenotypes, as well as other aspects of visual processing and development. This webinar will include the following:More details
Host
Speaker
VP Marketing and Sales at MaxWell Biosystems
Application Scientist at MaxWell Biosystems
Functional characterization of human iPSC-derived neurons at single-cell resolution
April 23, 2020 | 5pm (CET), 8am (PST), 11am (EDT)
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Dr. Marie Obien Dr. Michele Fiscella Abstract Recent developments in induced pluripotent stem cell (iPSC) technology have enabled easier access to human cells in vitro. With increasing availability of human iPSC-derived neurons, both healthy and disease cell lines, screening compounds for neurodegenerative diseases on human cells can potentially be performed in the earlier stages of drug discovery. To accelerate the functional characterization of iPSC-derived neurons and the effect of compounds, reproducible and relevant results are necessary. In this webinar, the speakers will:More details
Host
Speaker
VP Marketing and Sales at MaxWell Biosystems
VP Scientific Affairs at MaxWell Biosystems