Conference 2024 Presented Poster

Title

Next-Generation Electrophysiology for Functional Characterization of Human Neural Organoids 

In this poster you can learn more about how you can:

• Identify & isolate active areas of a 3D cellular model using a powerful user-friendly approach
• Measure the electrical activity of self-organizing in vitro cellular models
• Characterize iPSC-derived neural organoids at subcellular resolution

Abstract

Mammalian organs, such as the brain, are challenging to study being inaccessible to direct optical observation and experimental manipulation. However, recent advances in stem cell research allowed to develop novel three-dimensional (3D) culture techniques, such as neural organoids, resembling cell type diversity, developmental processes and function of human brain. Measuring the electrical activity of self-organizing in vitro cellular models in real time and label-free add valuable insights into the complexity of their functional structure. In this poster, we show that MaxWell Biosystems high-density microelectrode array (HD-MEA) technology allow to capture live the fast propagating extracellular action potentials in neural organoids at different scales, from network through single-neuron with high spatio-temporal resolution, low noise, and great flexibility. Our HD-MEA platforms, together with the extracted parameters highlighted in this poster, provide an uniquely powerful user-friendly approach for identifying and isolating active areas of a 3D cellular model in both acute recordings and longitudinal studies. This not only allows long-term disease modeling but also efficient compound testing on stem cell-derived in-vitro models.

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