Every Cell Counts.
MxW Bulletin | Edition No. 1 | 2020

Editor's Note

Hello Everyone! 

I'm writing this note in Zurich for the first time, while looking at late February snow falling outside. The beginning of 2020 couldn't be more exciting! We are preparing for several workshops and events throughout the year, so stay tuned for our announcements when we'll be in your area.

Central to our platforms, MaxOne and MaxTwo, is the software. MaxLab Live is the topic of this newsletter—highlighting the capability to not only visualize rich datasets from your samples, but more importantly, to perform experiments and run the analyses easily. Our aim is to enable you to get feedback about your cells' activity directly so you can achieve your research goals faster.

Here are the contents of this newsletter:
Enjoy reading!  :-)


MaxLab Live

Visualize, record and analyze your neuronal data
in just a few clicks

Do you have your sample - being iPS-derived or primary neuronal cell culture, brain slice, retina or organoid - prepared on your MaxOne or MaxTwo wells? Just start the MaxLab Live software to execute your experiments and generate results quickly.


As a live-cell activity imaging platform, MaxLab Live can display both the spiking dynamics of neuronal activity and propagation of network activity–label-free! Different visualizations allow to observe the activity of your sample, based on the information that you would like to get. 

  • Electrodes View: For 2D mapping of firing and bursting patterns
  • Traces View: For observing spike and burst shapes in detail
  • Raster View: For assessing synchronous firing 


You can perform your experimental workflow in just a few clicks. Start the Activity Scan to identify the location of active neurons on the electrode array. Different configurations can be chosen, depending on the needed spatial coverage and experiment duration. While the Activity Scan is running, set up the parameters for your next assay. It will automatically start once the activity scan is completed.

The Network Assay allows to continuously record the neurons identified from the Activity Scan.


The Analysis tab is your main dashboard for analyzing your recorded datasets.

MaxLab Live provides a solution for your entire experimental workflow. We are continuously working on advancing MaxLab Live to facilitate your electrophysiology experiments. If you want to learn more about the numerous other features of this powerful software, just contact us by email (info@mxwbio.com). We would be happy to answer any question! 

We are exhibiting at SOT 2020!
Come and meet us: Booth #683

User Interview: Dr. Maria Sundberg
Boston Children's Hospital

Dr. Maria Sundberg
Sahin Lab
Boston Children's Hospital

Human iPSC-derived dopaminergic neuron network that express Tyrosine hydroxylase (green) and TUJ1 (red)
(Credits: Dr. Maria Sundberg)

Hello Dr. Sundberg, could you please summarize your research for us?
I am working with dopaminergic neurons differentiated from human induced pluripotent stem cells (hiPSC) derived from patients exhibiting a rare genetic variation (16p11.2 CNV) . These patients present neuropathologies such as autism, bipolar disorder or schizophrenia. My goal is to study the phenotypes and genotypes of such neurons and to study how they respond to antipsychotic medications targeted to the dopamine neuroreceptors. 
In order to conduct this research, I am using different methods. One of them is electrophysiological recording using the MaxOne system. I also characterize the cells further using gene expression profiling to find out which genes are up-regulated and immunochemistry staining to quantify synapses, for example. 

Could you describe in more detail the way you are using MaxOne for your research?
I differentiate the cells in vitro for one month before plating them in the MaxOne wells. I then keep the cells for up to four weeks in culture and perform experiments every two days. I run the Activity Scan Assay to map the active areas and extract features such as firing rate and spike amplitude. I also perform the Network Assay to study bursting patterns and network development, before and after pharmacological treatment. I can extract such features from my culture thanks to the MaxLab Live Software. The old version of the software used to be more challenging as we were first using some coding to run the assay, but the latest software version allows to just start and run the assay with a few clicks. 

So is the MaxOne system helping you achieve your research goals?
The quality of the data that we can extract allows us to phenotype different cell lines and also characterize the effects of compounds. I also compared the system with low density microelectrode arrays. Even if the results between the platforms were reproducible, the high density of electrodes in the MaxOne wells allowed me to gain more information as it gave me the possibility to record every cell in the network. This is important for our work. Moreover, I can say that I learned a lot about my cells by using your system.

What is coming next?
Right now I am using MaxOne to study the dopaminergic neurons at the network level. Next, I would like to deepen the analysis at the cellular level by doing axon scanning and studying parameters such as the axonal conduction velocity.
We would also like to use the system for drug-screening purposes. In order to do this, we might even use the MaxTwo system in order to be able to perform more experiments in parallel.

Thank you very much for your time and all the best for what's next in your research!

MxW News

"Swiss Corner" at SLAS 2020
MaxWell Biosystems attended the SLAS 2020 conference on January 25-29 (https://www.slas2020.org/) in San Diego. The MxW Team met some of the 6'000 attendees from the high-throughput screening market. Marie Obien and Giulio Zorzi were happy to share the booth with SEED Biosciences, a Swiss start-up providing solutions for single cell assays  (https://seedbiosciences.com/).
New Team Members

In January, MxW welcomed Sophie Wolf-Demarche and Amanda Klaeger as new members of the team.

Sophie joined us as a Marketing and Communications Manager. Amanda is our new Application Scientist who will help supporting our valued customers. 

Next Conferences and Exhibitions

SOT/TOX 2020: Booth #683
15-19 March 2020 | Anaheim, California

Neurizons 2020:  Booth #3
26-29 May 2020 | Goettingen, Germany

World Pharma Week 2020: Booth #217
2-4 June 2020 | Boston, 

Latest Publications using MxW Technology

In Vivo
M. Kollo, R. Racz, M. Hanna, A. Obaid, M. R. Angle, W. Wray, Y. Kong, A. Hierlemann, J. Müller, N. A. Melosh, A. T. Schaefer, "CHIME: CMOS-hosted in-vivo microelectrodes for massively scalable neuronal recordings"bioRxiv 570069 (DOI: 10.1101/570069). Online
K. Shimba, T. Asahina, K. Sakai, Y. Jimbo, K. Kotani, "Evaluation of Conduction Properties of Sensory Axons with High-Density Microelectrode Array"12th Biomedical Engineering International Conference (BMEiCON)IEEE Conference Publication. (DOI: 10.1109/BMEiCON47515.2019.8990233). 
Brain Slice
M. Kajiwara, R. Nomura, F. Goetze, T. Akutsu, M. Shimono, "Inhibitory neurons are a Central Controlling regulator in the effective cortical microconnectome"bioRxiv 2020.02.18.954016 (DOI: 10.1101/2020.02.18.954016). Online
Cell Cultures, Neural Network
A. Masumori, L. Sinapayen, N. Maruyama, T. Mita, D. Bakkum, U. Frey, H. Takahashi, T. Ikegami, "Neural Autopoiesis: Organizing Self-Boundaries by Stimulus Avoidance in Biological and Artificial Neural Networks."Artificial Life 2020, p. 1–22 (DOI: 10.1162/artl_a_00314). Online
Physiology of Sleep
M. Bandarabadi, A. Vassalli, M. Tafti, "Sleep as a default state of cortical and subcortical networks"Current Opinion in Physiology 2020, 15, 60–67 (DOI: 10.1016/j.cophys.2019.12.004). Online

Cell Cultures, Networks
Y. Uwate, M. E. J. Obien, U. Frey, Y. Nishio, " Time Series Analysis of Neurons and Visualization of Network Characteristics"Proceedings of RISP International Workshop on Nonlinear Circuits, Communications and Signal Processing (NCSP'19) 2019, pp. 450-453 2019. Online