Every Cell Counts.
MxW Bulletin | Edition No. 2 | 2020

Editor's Note

Hello Everyone! 

We are all currently in an extraordinary situation. The coronavirus pandemic has affected the way we live and work. Home office has become the norm. Online and digital activities are now highly in-demand at MaxWell Biosystems, while our production and research & development proceed as much as possible. Remote training, support, and demos are all ongoing. This fast-changing situation has allowed us to rise up to the challenge, strengthen our teamwork, and deepen our dedication to help our customers achieve their goals.

For this newsletter, we highlight one of the key aspects for a successful MaxOne or MaxTwo experiment: the cell culture protocol. We have refined each step according to our own and other users' experiences. We do hope that as the labs start to operate again, this protocol can be helpful to speed up project restart and experiments can run smoothly from the get-go.

We are also very happy to feature one of our users, Elixirgen Scientific. Thanks a lot for our collaboration and for offering our readers a discount for their first purchase! 

Here are the contents of this newsletter: Stay safe and enjoy reading!  :-)


MxW Neuronal Cell Culture Protocol

In our previous newsletter, we presented how the MaxLab Live software provides a solution for your entire experimental workflow, from cell activity visualization to data analysis. To ensure the reproducibility of your experiments and to obtain meaningful results, it is crucial to have a well-established cell culture protocol.

We have improved our cell culture protocol applicable for MaxOne and MaxTwo. The protocol has been developed for primary and induced pluripotent stem cell (iPSC)-derived neurons. Other types of cells may be cultured similarly, with some adjustments in the cell density, culture media, and surface coating. If you have any questions, you can schedule a session with one of our application scientists to discuss your experimental plans by following this link:

Download Protocol


User Application Protocol

Elixirgen Scientific, a biotech company focused on stem cell related technology is using the MaxTwo Multi-Well High-Density Microelectrode Array System to characterize iPSC-derived neurons.

If you are interested to establish a protocol for plating your cells on MaxOne and MaxTwo and to see exciting results, contact us at info@mxwbio.com.
Numerous conferences have been cancelled due to the COVID-19 crisis. While we are currently not able to deliver scientific talks and meet you at conferences, we decided to run live webinars instead. We had a successful first session with hundreds of live attendees on April 23rd about "Functional characterization of human iPSC-derived neurons at single-cell resolution". If you missed it or if you would like to watch it again, click the button below. 
Watch Replay

User Interview: Dr. Tetsuya Tanaka,  Elixirgen Scientific

Dr. Tetsuya Tanaka (contact)
Senior Scientist
Elixirgen Scientific

Dr. Tanaka's Family
(Credits: Dr. Tetsuya Tanaka)

Hello Dr. Tanaka, could you please summarize your research for us?

At Elixirgen Scientific, we have developed our Quick-TissueTM technology that accelerates and removes the complexity from the stem cell differentiation process. It’s our mission to ensure that this technology can reach every lab, so that induced pluripotent stem cells (iPSCs) can be the enabling innovation they were meant to be — accelerating advancements in human health through more accurate human cell-based models. Our technology uses a cocktail of essential transcription factors to drive rapid stem cell differentiation in as little as 10 days without introducing a genetic footprint. Our portfolio includes iPSC-derived cells, differentiation kits, and services for a variety of neuronal cell types, including GABAergic, dopaminergic, cholinergic, and mixed neurons with more cell types on the way.

Could you describe the way you are using MaxTwo for your research?

Our main purpose of using MaxTwo is to assess the quality and maturation of the human iPSC-derived neurons we produce using our Quick-TissueTM technology. Our recent data using MaxTwo show that our Mixed Neurons, which are a mixed population of neuron subtypes, fire synchronized bursts within two weeks when co-cultured with human primary astrocytes as detailed in our MaxTwo Application Protocol

Which specific feature(s) do you like most about MaxTwo?

Unlike other commercially available MEA systems, MaxTwo has an outstanding capability to measure and track action potentials in single neurons due to how the electrodes are arrayed in such a high density manner. Because of this feature, even if neurons aggregate locally, which is one of the major problems in MEA assays, neurons are likely making contacts with some of the electrodes and so action potentials can be measured effectively. By measuring the velocity that the action potential propagates through single axons, the maturity of single neurons can be quantitatively defined. Potentially, users would be able to differentiate action potentials measured on axons from ones on dendrites, which is impossible with other MEA systems.

Have there been any challenges and how did MaxWell Biosystems help to resolve them?

We started to implement MEA assays in our quality assurance program about one year ago and our experience with MEA data analysis was very limited at the time. Although the software that comes with MaxTwo is easy to handle, the support we receive from the MaxWell Biosystems team has always been quick and comprehensive. We have learned a lot about data acquisition and analysis through our interactions with the MaxWell Biosystems support team.

Is there anything else you would like to share with our readers?

We are quite pleased to have MaxTwo in our facility. At Elixirgen Scientific, we want to change how researchers model human biology and disease. We believe that MaxTwo will help us identify the unique features of human neurons that cannot be substituted by rodent neurons and ultimately help us achieve our goal of accelerating human health by developing a more accurate model system.

MxW Blog

MxW Team: Ski-Day
Beginning of March, we had the chance to enjoy a day together in the beautiful ski resort of Flumserberg. Today, it feels far away and we are very glad that we could enjoy some time all together before the lockdown. Read more about it in our blog.
>> Link to post
MxW Team: Work from Home Experiences

Like many others, we contribute to protect those at risk and help to slow down the spread of COVID-19 by working from home since beginning of March. Read more about how MaxWell Biosystems employees make the best of this extraordinary situation in our blog.
>> Link to post

Latest Publications using MxW Technology

In Vivo
A. Obaid, M.-E Hanna, Y.-W. Wu, M. Kollo, R. Racz, M. R. Angle, J. Müller, N. Brackbill, W. Wray, F. Franke, E. J. Chichilnisky, A. Hierlemann, J. B. Ding, A. T. Schaefer, N. A. Melosh, "Massively parallel microwire arrays integrated with CMOS chips for neural recording." Science Advances, 2020, 6(12), eaay2789 (DOI:10.1126/sciadv.aay2789). Online