MaxTwo

Maximize your cell functional assays

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MaxTwo Multi-Well High-Density Microelectrode Arrays (HD-MEAs) represent a next-generation electrophysiology platform for higher-throughput applications. The system enables precise recording and stimulation of electrogenic cells across multiple wells, combining single-cell sensitivity with scalable experimental capacity. MaxTwo captures electrical activity from individual cells and complex networks with high precision, allowing scientists to detect even the smallest action potentials. This accelerates data collection and deepens insight into cellular function.

MaxTwo 
key features

Our Technology

Scale-up your experiments

Discover the full potential of HD-MEAs at throughput: scalable, precise, and designed for automation.

Consistent high resolution in every well

Increase throughput without compromising data quality. Each well features 26,400 electrodes at a density of 3,265 electrodes/mm², maintaining single-cell and subcellular resolution across the entire plate.

Automation-tested platform

Engineered for compatibility with standard laboratory automation tools. MaxTwo is designed for seamless integration with liquid handlers, plate movers, and robotic systems, enabling plug-and-play operation for automated workflows.

Efficient large-scale data handling

Manage high volumes of recordings with ease. Sample annotation, batch processing and report generation streamline analysis across multiple wells and experiments, accelerating insight and decision-making.

Fully integrated perfusion system

Control experimental conditions across all wells with the first fully integrated perfusion system in a MEA multi-well platform. Maintain sample viability with minimal noise while recording from all wells in parallel.

Unlock the full potential of each sample

Confirm what you expected and reveal what was never visible before through high sensitivity, targeted stimulation, and novel phenotypic readouts.

Capture every signal at its source with high sensitivity

With a dense electrode matrix in every well, the fine-pitch array lets you target any cell in a network. MaxTwo records at microvolt-level noise and microsecond resolution, enabling the detection of subtle phenotypes and the smallest signals that may otherwise go undetected.

Novel phenotypic readouts at scale

Track axons non-invasively, for the first time at throughput, across hundreds of neurons. MaxTwo enables long-term, label free recording and analysis to reveal functional phenotypes and developmental changes over time.

Targeted electrical stimulation

Access advanced stimulation capabilities to investigate functional connectivity and hidden network dynamics. Precise electrode targeting allows for reproducible stimulation of your sample.

Secure reproducibility in every well

Guarantee meaningful results and experimental stability on a precision-engineered platform.

Always at the right spot

With 26,400 densely packed microelectrodes in every well, a contact is always exactly where the activity occurs. Dense, flexible coverage provides representative sampling of the entire culture, capturing events for statistically robust analysis and delivering high-quality, reproducible results every time.

Standardized workflow

Follow our validated plating and maintenance protocols optimized for a wide range of applications. Using standardized step-by-step guidelines removes uncertainty, enhances consistency, and lets you compare datasets with confidence.

Hardware and environmental stability

Rely on biological variation - not hardware drifts. Precision-molded, function-tested MaxTwo's multi-well plates ensure reliable performance and consistency across every well. The system's active temperature regulation plus controlled CO₂ keep culture conditions stable from run to run. Together they lock in stable, high-quality signal acquisition over repeated recordings, so your longitudinal studies remain truly reproducible.

Relevant
Applications 

Functional Phenotyping
Disease Modeling
Pharmacology & Toxicology
Method Development

Relevant
Biological Models

Neuronal Cell Cultures
Organoids
Brain Slices

Technical
Specifications

One-button Interface
Open/close the cover
Turn on/off the system
Lock the well plate
System Status Indicator
LED
Dimension (L x W x H)
40x16x12 cm3
Well-Plate Compatibility
6- and 24-Well Plate
Temperature Control
Yes
CO2 Control
Yes, via gas mixer

MaxTwo Well Plates

At the core of every MaxTwo System are our HD-MEA Multi-Well plates, which capture even the most subtle electrical signals down to sub-cellular resolution, label-free. Learn more in our Technology section.

Each well is equipped with a complete HD-MEA chip featuring 26’400 small, sensitive electrodes. This design delivers outstanding resolution, low noise, and flexible experimental possibilities for consistent, reproducible results across samples.

The Multi-Well format allows for significantly increased throughput, for the direct comparison of different phenotypes and treatments, and it makes the most of precious samples such as organoids and iPSC-derived neurons.

Need help choosing? Check the spec table below or contact our experts.

Plate Specifications

Features
6-Well Plate
24-Well Plate
Well-Plate dimension (L x W x H)

127.8 x 85.8 x 16.1 mm3

127.8 x 85.8 x 16.1 mm3

Number of wells

6

24

Well (inner) diameter

29.0 mm

15.3 mm

Active sensing area

3.85 x 2.10 mm2

3.85 x 2.10 mm2

Total number of electrodes

6 x 26ʼ400

24 x 26ʼ400

Electrode density

3’265 electrodes/mm2

3’265 electrodes/mm2

Electrode center-to-center distance

17.5 µm

17.5 µm

Electrode size

12.0 x 8.8 µm2

11.5 x 11.5 µm2

Electrode material

Platinum Black

Platinum

Surface topography

<2.0 µm

<0.3 µm

Sampling rate

10.0 kHz/channel

10.0 kHz/channel

Number of recording channels

1ʼ020/well

1ʼ020/well

Typical noise per well

3.6 µVrms

3.8 µVrms

Electrical stimulation

Yes

No

Simultaneous recordings

All 6 wells in parallel

6 wells in parallel, row by row

Electronic ID

No

Yes

Sterilization treatment

No

EtO treatment according to EN ISO 11737-2

MaxTwo Accessories

MaxTwo Perfusion System

Discover the groundbreaking MaxTwo Perfusion System, the first fully integrated multi-well MEA perfusion system on the market. It enables continuous, parallel medium flow to every well, keeping culture conditions stable during acute experiments. This consistent perfusion preserves the sample's health and ensures high-quality recordings across all wells for the duration of the entire experiment.

MaxTwo Perfusion System Features

Dimension (LxWxH)
63 x 39 x 25 cm3
Well-plate compatibility
6-Well Plate
Perfusion rate
0.04 - 3.00 mL/min
Medium temperature
Room temperature - 60 °C
Medium volume
500 mL with bottle heater
100 mL - 10 L with in-line heater
Autoclavable components
Cartidges, tubings
Product Documentation

MaxTwo Guide

Optimize your experiments with the MaxTwo User Guide: step-by-step setup, safety, plate handling, maintenance, and troubleshooting for the MaxTwo HD-MEA system.

Brochures

MaxTwo Brochure

Unlock the potential of MaxTwo, a multiwell HD-MEA system offering unmatched resolution, high throughput, and exceptional sensitivity to maximize cell functional assays.

Brochures

MaxTwo Perfusion System Brochure

Introducing the first fully integrated multiwell MEA perfusion system, MaxTwo Perfusion system ensures continuous medium flow and high-resolution data for advanced cell research.

X. Shan et al.

Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation

Cell Reports Methods
|
2024
A. B. Kesdoğan, A. Neureiter, A. J. Gaebler, A. K. Kalia, J. Körner, and A. Lampert

Analgesic effect of Botulinum toxin in neuropathic pain is sodium channel independent

Neuropharmacology
|
2024
W. Lin et al.

Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons

iScience
|
2023

Dr. Tetsuya Tanaka

Ricoh Biosciences, Inc., Baltimore, Maryland, USA

“Our main purpose of using MaxTwo is to assess the quality and maturation of the human iPSC-derived neurons we produce using our Quick-TissueTM technology. Unlike other commercially available MEA systems, MaxTwo has an outstanding capability to measure and track action potentials in single neurons due to how the electrodes are arrayed in such a high density manner."

Dr. Maria Sundberg

Sahin Lab, Neurology Department Boston Children’s Hospital/Harvard Medical School, USA

“The multi-well platform allows us to record from several wells/conditions at the same time. This is helpful when comparing the network function between control and patient cell populations, and also when comparing drug treated vs vehicle treated neuronal networks functionality at the same time.”

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