A biocompatible multi-electrode cell for the study of neurons using capillary microscopy

Modern in vitro studies of neural networks require the integration of electrophysiological methods and high-precision imaging. Despite advances in the development of multielectrode arrays (MEA), issues remain regarding their compatibility with optical methods. This is particularly important for long-term experiments requiring continuous observation of living cells. For these purposes, a biocompatible multielectrode cell creating controlled conditions for cultivating neurons during experiments was developed. This article describes an integrated system compatible with a scanning capillary microscope. The cell developed here ensures parameter stability by means of interconnections between subsystems, i.e., gas and temperature control, along with microfluidic management of buffer solutions.