Abstract
Details
- Guided differentiation enables spinal cord organoid generation: Human iPSC-derived organoids recapitulate key developmental processes and dorsoventral patterning.
- Diverse neural cell types are formed: Organoids contain motor and sensory neurons, astrocytes, and oligodendrocytes showing progressive maturation.
- Region-specific gene expression emerges: Expression of HOXA4, HOXA5, OLIG2, HB9, and FOXP1 reflects proper spinal cord regionalization.
- Spontaneous network activity develops: Organoids exhibit synchronized neuronal firing recorded acutely with MaxWell Biosystems’ MaxTwo 6-well plate and Liquid Holder.
- Powerful model for research and screening: Suitable for studies of human neurodevelopment, disease modeling (e.g., ALS, SMA), and drug discovery.