In this Application Protocol, we describe how Elixirgen Scientific's human iPSC-derived Mixed Neurons can be thawed, plated, and maintained on the MaxWell Biosystems MaxTwo Multiwell multielectrode array (MEA) system for non-invasive, label-free measurement of neuronal activity. The Mixed Neurons fire synchronized bursts within 2 weeks after plating when co-cultured with human primary astrocytes.