Abstract
Details
- Semi-automated HD-MEA workflow established: The MaxTwo 24-Well Plate combined with the VIAFLO 96 enables streamlined cell plating, and parallel media exchange and compound administration with reduced hands-on time and operator-independent handling.
- High-throughput functional profiling demonstrated: Human iPSC-derived glutamatergic neuron–astrocyte co-cultures were maintained for over 4 weeks with twice-weekly electrophysiological recordings using ActivityScan and Network Assays.
- Highly reproducible recordings across 24 wells: Parallel liquid handling and low-noise HD-MEA acquisition resulted in <10% CV across key activity and network metrics.
- Robust pharmacology validation performed: Tetrodotoxin (TTX) application using partial tip loading reliably silenced network activity across treated wells, with full recovery after washout.
- Scalable platform for screening applications: The combined system enables high-throughput phenotyping, comparative studies, and compound testing with precise delivery and sensitive detection of functional phenotypes.