Nikon Application Note

High-density microelectrode arrays (MaxOne and MaxTwo MultiwellHD-MEAs, MaxWell Biosystems) are platforms for electrophysiology research that enable simultaneous recording and stimulation of electrogenic cells such as retinal ganglion cells, cardiomyocytes, human induced pluripotent stem cell (iPSC)-derived cells, brain slices, and brain organoids. These models have extensive applications in neuroscience research, drug discovery, cell analysis and so on. However, these arrays block transmitted light and prevent the use of label-free live cell imaging techniques such as phase contrast microscopy. Therefore, it is necessary to fix the cells and perform immuno- or dye staining to observe them. This application note introduces a case study wherein human iPSC-derived neuronal cells were seeded on the arrays and imaged with the ECLIPSE LV100ND upright microscope, enabling the assessment of neuronal cell attachment to the array through analysis utilizing the AI technology of NIS-Elements software.