MaxWell Webinar with FUJIFILM Cellular Dynamics

Human induced pluripotent stem cell (hiPSC)-derived neurons are a powerful asset for in vitro modeling of neurodegenerative diseases. These cells have become increasingly accessible as commercial manufacturing at FUJIFILM Cellular Dynamics has established itself as the gold-standard and consistent supplier for biopharma and academic research. Electrophysiological assays such as manual patch clamp and microelectrode array (MEA) are essential for studying iPSC-derived neuronal function and are a useful tool for identifying disease phenotypes when comparing iPSC-derived neurons derived from healthy donors to iPSCs engineered to contain risk-associated disease mutations, or

patient lines containing risk-associated disease mutations. This webinar will focus on advancements and technical advantages for using iPSC-derived neurons from FUJIFILM Cellular Dynamics on this platform. Case studies will be presented modeling Parkinson’s disease (LRRK2 & GBA mutant iCell DopaNeurons), Amyotrophic lateral sclerosis (TDP43 mutant iCell Motor Neurons), and Frontotemporal Dementia (GRN mutation iCell Induced Excitatory Neurons). These case studies will highlight the benefits for profiling a panel of risk-associated disease models on HD-MEA, the importance of isogenic controls, and exciting new mutation-containing neurons (TMEM175 mutant iCell DopaNeurons). Technical considerations and assay parameters for detecting disease phenotypes on the HD-MEA will be discussed, showcasing how neuronal spike activity and network bursting can be impacted by the type of neurons tested, composition of the culture media and supplements used, disease genotype, and 2D vs 3D co-cultures.