Neuronal Networks

Neuronal Networks

Primary Cell Cultures on MaxOne MEA

Dissociated cell cultures can be plated, grown, and imaged on MaxOne MEAs. The figure shows an overlay of multiple confocal stack images: cell bodies and neurites are visible on the left side and the electrode array is focused on the right side.

What You Can Do

Single Cell Level Analysis

Isolate extracellular action potentials from individual cells easily.

Select electrodes with the best signals from each cell and improve your spike sorting.

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Analyze subcellular features, such as full axonal arbors in single neurons.

Electrical signals tracked along neurites enable to investigate novel parameters, difficult to obtain with previous technologies.

 
High-resolution axonal action potential propagation tracking

The video shows the propagation of an AP through the axonal arbor of a single neuron. Electrical signals tracked along neurites enable to investigate novel parameters, difficult to obtain with previous technologies.

Axonal branch signals tracking
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The video shows the propagation of a stimulation-induced action potential through a branching segment of an axon. To reduce noise and better reveal the action potential, 300 trials have been averaged.

Network Level Analysis

Record and/or stimulate every cell on the MEA.

MaxOne allows simultaneous recording from thousands of cells yields rich datasets for studying neuronal networks. Stimulation experiments can be designed with high flexibility to manipulate network dynamics.

Neuronal Networks 4

Electrically image active cells on the MEA.

Localize active cells on the MEA during experiments. The optical image from fluorescence microscopy matches the electrical image obtained using MaxOne.

Neuronal Networks 5
Electrical image Neuronal Networks

Study cells and networks across different spatial and temporal scales.

Action potentials and local field potentials can be simultaneously recorded by MaxOne over time scales of microseconds to months. The raster plot shows the dynamics of network activity using 1,024 active electrode sites.

Raster plot Neuronal Networks

Selected Publications

DeePhys: A machine learning–assisted platform for electrophysiological phenotyping of human neuronal networks

Hornauer, Philipp; Prack, Gustavo; Anastasi, Nadia; Ronchi, Silvia; Kim, Taehoon; Donner, Christian; Fiscella, Michele; Borgwardt, Karsten; Taylor, Verdon; Jagasia, Ravi; Roqueiro, Damian; Hierlemann, Andreas; Schröter, Manuel

DeePhys: A machine learning–assisted platform for electrophysiological phenotyping of human neuronal networks Journal Article

Stem Cell Reports, 2024.

Abstract | Links | BibTeX

Hyperexcitability and translational phenotypes in a preclinical model of SYNGAP1mutations

Silverman, Jill L; Fenton, Timothy; Haouchine, Olivia; Hallam, Elizabeth; Smith, Emily; Jackson, Kiya; Rahbarian, Darlene; Canales, Cesar; Adhikari, Anna; Nord, Alex; Ben-Shalom, Roy

Hyperexcitability and translational phenotypes in a preclinical model of SYNGAP1mutations Journal Article

Research Square, 2023.

Abstract | Links | BibTeX

Critical dynamics arise during structured information presentation within embodied in vitro neuronal networks

Habibollahi, Forough; Kagan, Brett J; Burkitt, Anthony N; French, Chris

Critical dynamics arise during structured information presentation within embodied in vitro neuronal networks Journal Article

Nature Communications, 2023.

Abstract | Links | BibTeX

Functional imaging of conduction dynamics in cortical and spinal axons

Radivojevic, Milos; Punga, Anna Rostedt

Functional imaging of conduction dynamics in cortical and spinal axons Journal Article

eLife, 2023.

Abstract | Links | BibTeX

Investigation of the input-output relationship of engineered neural networks using high-density microelectrode arrays

Duru, Jens; Maurer, Benedikt; Doran, Ciara Giles; Jelitto, Robert; Küchler, Joël; Ihle, Stephan J; Ruff, Tobias; John, Robert; Genocchi, Barbara; Vörös, János

Investigation of the input-output relationship of engineered neural networks using high-density microelectrode arrays Journal Article

Biosensors and Bioelectronics, 2023.

Abstract | Links | BibTeX

Microfluidic technologies for reconstituting neuronal network functions in vitro

Yamamoto, Hideaki; Hirano-Iwata, Ayumi; Sato, Shigeo

Microfluidic technologies for reconstituting neuronal network functions in vitro Journal Article

JSAP Review, 2023.

Abstract | Links | BibTeX

Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules

Girardi, Gregory; Zumpano, Danielle; Goshi, Noah; Raybould, Helen; Seker, Erkin

Cultured Vagal Afferent Neurons as Sensors for Intestinal Effector Molecules Journal Article

biosensors, 2023.

Abstract | Links | BibTeX

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

Xu, He Jax; Yao, Yao; Yao, Fenyong; Chen, Jiehui; Li, Meishi; Yang, Xianfa; Li, Sheng; Lu, Fangru; Hu, Ping; He, Shuijin; Peng, Guangdun; Jing, Naihe

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells Journal Article

Cell Regeneration, 2023.

Abstract | Links | BibTeX

Multiplex epigenome editing of MECP2 to rescue Rett syndrome neurons

Qian, Junming; Guan, Xiaonan; Xie, Bing; Xu, Chuanyun; Niu, Jacqueline; Tang, Xin; Li, Charles H; Colecraft, Henry M; Jaenisch, Rudolf; Liu, Shawn X

Multiplex epigenome editing of MECP2 to rescue Rett syndrome neurons Journal Article

Science Translational Medicine, 2023.

Abstract | Links | BibTeX

CASK loss of function differentially regulates neuronal maturation and synaptic function in human induced cortical excitatory neurons

McSweeney, Danny; Gabriel, Rafael; Jin, Kang; Pang, Zhiping P; Aronow, Bruce; and Pak, ChangHui

CASK loss of function differentially regulates neuronal maturation and synaptic function in human induced cortical excitatory neurons Journal Article

iScience, 2022.

Abstract | Links | BibTeX

Long-term morphological and functional dynamics of human stem cell-derived neuronal networks on high-density micro-electrode arrays

Habibey, Rouhollah; Striebel, Johannes; Schmieder, Felix; Czarske, Jürgen; Busskamp, Volker

Long-term morphological and functional dynamics of human stem cell-derived neuronal networks on high-density micro-electrode arrays Journal Article

Frontiers in Neuroscience, 2022.

Abstract | Links | BibTeX

A multi-modal fitting approach to construct single-neuron models with patch clamp and high-density microelectrode arrays

Buccino Alessio Paolo; Damart, Tanguy; Bartram Julian; Mandge Darshan; Xue Xiaohan; Zbili Mickael; Gänswein Tobias; Jaquier Aurélien; Emmenegger Vishalini; Markram Henry; Hierlemann Andreas; Van Geit Werner.

A multi-modal fitting approach to construct single-neuron models with patch clamp and high-density microelectrode arrays Journal Article

bioRxiv, 2022.

Abstract | Links | BibTeX

Inferring monosynaptic connections from paired dendritic spine Ca2+ imaging and large-scale recording of extracellular spiking

Xue, Xiaohan; Buccino, Alessio Paolo; Kumar, Sreedhar Saseendran; Hierlemann, Andreas; Bartram, Julian

Inferring monosynaptic connections from paired dendritic spine Ca2+ imaging and large-scale recording of extracellular spiking Journal Article

Journal of Neural Engineering, 2022.

Abstract | Links | BibTeX

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells

Xu, Jax H; Yao, Yao; Yao, Fenyong; Chen, Jiehui; Li, Meishi; Xianfa, ; Yang, ; Li, Sheng; Lu, Fangru; Hu, Ping; He, Shuijin; Peng, Guangdun; Jing, Naihe

Generation of functional posterior spinal motor neurons from hPSCs-derived human spinal cord neural progenitor cells Journal Article

BioRxiv, 2022.

Abstract | Links | BibTeX

Unifying framework for information processing in stochastically driven dynamical systems

Kubota, Tomoyuki; Takahashi, Hirokazu; and Nakajima, Kohei

Unifying framework for information processing in stochastically driven dynamical systems Journal Article

Physical Review Research, 2021.

Abstract | Links | BibTeX

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