MaxTwo Multiwell HD-MEA System

MaxTwo High-Density Microelectrode Array (HD-MEA) System

Key Features

High-Quality Data
High-resolution and high-quality data while tracking dynamic changes at cellular, subcellular and network levels.
Sensitivity
Smallest signals capture (uV) thanks to low-noise recording channels and high electrode density.
Throughput
Recording from 6 and 24 wells, increasing test capabilities and decreasing experimental time, while ensuring consistency across wells.
Reproducibility
Optimized recordings strategies to analyze the entire culture at individual neuronal levels, increasing data reproducibility and statistical power.
Long-Term Experiments
Cell development, maturation, or compound effects access by performing longitudinal experiments over the course of days, weeks, and months.
Integrity
Non-invasive and label-free recordings, eliminating any potential side effects associated with the use of dyes and prolonged exposure to light.

Product Overview

MaxTwo HD-MEAs represent the next-generation electrophysiology platform, featuring densely packed microelectrodes capable of capturing the electrical signature of electrogenic cells with unprecedented precision. Its exceptional data quality enables you to drive fast and meaningful conclusions in our cell-based assays. 

Featuring 26’400 electrodes within a large sensor area of 3.85 x 2.10 mm2 per well, MaxTwo HD-MEAs measure the activity of cells across multiple scales – capturing whole network dynamics and identifying single cell function. With high electrode resolution and low noise, spikes generated by any neuron on the array can be picked up by an electrode in close vicinity. Such sensitivity enables to detect cells’ small signals, such as induced pluripotent stem cell (iPSC)-derived neurons and propagating action potentials along axonal arbors. 

26’400 Pt-Electrodes Per Well
3’625 Electrodes/mm2
Low-Noise Readouts
Flexible Electrical Stimulation
Different readouts
Network level
Cellular level
Subcellular level
MaxTwo System Features
One-button interfaceOpen/close the cover
Turn on/off the system
Lock the well plate
System status indicator LED
Dimension (LxWxH)40 x 16 x 12 cm3
Well-Plate compatibility6- and 24-Well Plate
Temperature controlYes
CO2 controlYes, via gas mixer

MaxTwo HD-MEA System is compatible with 6- and 24-Well Plates, increasing test capabilities and decreasing experimental time, while ensuring consistency across wells.

Learn About MaxTwo Well Plates
MaxOne HD-MEA System is a high-density microelectrode array (HD-MEA) system in a single-well format. It can operate inside cell-culture incubators. Accessories are available for perfusion-compatibility. Microscopy of samples on the HD-MEA surface can be performed using upright objectives.
MaxLab Live Software is a comprehensive and user-friendly tool designed for visualizing, recording, and analyzing data recorded with MaxOne and MaxTwo. Its assays guide the user through automated workflows to the extraction of key metrics at multiple levels, including network, single-cell, and subcellular details.
Recapitulation of Perturbed Striatal Gene Expression Dynamics of Donor’s Brains With Ventral Forebrain Organoids Derived From the Same Individuals With Schizophrenia

Sawada, Tomoyo; Barbosa, André R; Araujo, Bruno; McCord, Alejandra E; D’Ignazio, Laura; Benjamin, Kynon J M; Sheehan, Bonna; Zabolocki, Michael; Feltrin, Arthur; Arora, Ria; Brandtjen, Anna C; Kleinman, Joel E; Hyde, Thomas M; Bardy, Cedric; Weinberger, Daniel R; Paquola, Apuã C M; Erwin, Jennifer A

Recapitulation of Perturbed Striatal Gene Expression Dynamics of Donor’s Brains With Ventral Forebrain Organoids Derived From the Same Individuals With Schizophrenia Journal Article

American Journal of Psychiatry, 2023, ISSN: 0002-953X.

Abstract | Links | BibTeX

Potentiating NaV1.1 in Dravet syndrome patient iPSC-derived GABAergic neurons increases neuronal firing frequency and decreases network synchrony

Kelley, Matt R; Chipman, Laura B; Asano, Shoh; Knott, Matthew; Howard, Samantha T; Berg, Allison P

Potentiating NaV1.1 in Dravet syndrome patient iPSC-derived GABAergic neurons increases neuronal firing frequency and decreases network synchrony Journal Article

bioRxiv, 2023.

Abstract | Links | BibTeX

Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons

Lin, Waka; Shiomoto, Shusaku; Yamada, Saki; Watanabe, Hikaru; Kawashima, Yudai; Eguchi, Yuichi; Muramatsu, Koichi; Sekino, Yuko

Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons Journal Article

iScience, 2023.

Abstract | Links | BibTeX

Multiplex epigenome editing of MECP2 to rescue Rett syndrome neurons

Qian, Junming; Guan, Xiaonan; Xie, Bing; Xu, Chuanyun; Niu, Jacqueline; Tang, Xin; Li, Charles H; Colecraft, Henry M; Jaenisch, Rudolf; Liu, Shawn X

Multiplex epigenome editing of MECP2 to rescue Rett syndrome neurons Journal Article

Science Translational Medicine, 2023.

Abstract | Links | BibTeX

Predicting in vitro single-neuron firing rates upon pharmacological perturbation using Graph Neural Networks

Kim, Taehoon; Chen, Dexiong; Hornauer, Philipp; Emmenegger, Vishalini; Bartram, Julian; Ronchi, Silvia; Hierlemann, Andreas; Schröter, Manuel; Roqueiro, Damian

Predicting in vitro single-neuron firing rates upon pharmacological perturbation using Graph Neural Networks Journal Article

Frontiers in Neuroinformatics, 2023.

Abstract | Links | BibTeX

Downregulation of PMP22 ameliorates myelin defects in iPSC-derived human organoid cultures of CMT1A

Lent, Jonas Van; Vendredy, Leen; Adriaenssens, Elias; Authier, Tatiana Da Silva; Asselbergh, Bob; Kaji, Marcus; Weckhuysen, Sarah; Bosch, Ludo Van Den; Baets, Jonathan; Timmerman, Vincent

Downregulation of PMP22 ameliorates myelin defects in iPSC-derived human organoid cultures of CMT1A Journal Article

Brain, 2022.

Abstract | Links | BibTeX

Homophilic wiring principles underpin neuronal network topology in vitro

Akarca, Danyal; Dunn, Alexander W E; Hornauer, Philipp J; Ronchi, Silvia; Fiscella, Michele; Wang, Congwei; Terrigno, Marco; Jagasia, Ravi; Vértes, Petra E; Mierau, Susanna B; Paulsen, Ole; Eglen, Stephen J; Hierlemann, Andreas; Astle, Duncan E; Schröter, Manuel

Homophilic wiring principles underpin neuronal network topology in vitro Journal Article

BioRxiv, 2022.

Abstract | Links | BibTeX

P97/VCP ATPase inhibitors can rescue p97 mutation-linked motor neuron degeneration

Wang, F; Li, S; Wang, T Y; Lopez, G A; Antoshechkin, I; Chou, T F

P97/VCP ATPase inhibitors can rescue p97 mutation-linked motor neuron degeneration Journal Article

Brain Communications, 2022.

Abstract | Links | BibTeX

Functional imaging of brain organoids using high-density microelectrode arrays

Schröter Manuel; Wang, Congwei; Terrigno Marco; Hornauer Philipp; Huang Ziqiang; Jagasia Ravi; Hierlemann Andreas

Functional imaging of brain organoids using high-density microelectrode arrays Journal Article

MRS Bulletin, 2022.

Abstract | Links | BibTeX

Corticotropin-releasing hormone (CRH) alters mitochondrial morphology and function by activating the NF-kB-DRP1 axis in hippocampal neurons

Battaglia, Chiara R; Cursano, Silvia; Calzia, Enrico; Catanese, Alberto; Boeckers, Tobias M

Corticotropin-releasing hormone (CRH) alters mitochondrial morphology and function by activating the NF-kB-DRP1 axis in hippocampal neurons Journal Article

Cell Death & Disease, 2020.

Abstract | Links | BibTeX

Would you like to learn more about MaxTwo? Book a one-to-one call with one of our scientist to discuss how MaxWell Biosystems’ high-content electrophysiology solutions can bring new key insights to your project or request a quote.

We provide training and support on all aspects of the MaxTwo platform. We have the expertise to help you design experiments and/or analysis tools and integrate MaxTwo with your automation system.

Ask a Question
Contact Us






Albisriederstrasse 253
8047 Zurich, Switzerland
+41 44 244 24 24
Schedule a call
info@mxwbio.com
Subscribe to our Newsletter