MaxOne for Brain Slice Studies
MaxOne enables researchers to perform label-free analysis of intact brain networks in vitro:
- Acute brain slices
- Organotypic brain slice cultures and organoids
- Ex-vivo brain preparation (e.g., eye-brain from turtles)
Sample Acute Brain Slice Preparation for Recording
- After dissection and slicing, incubate the brain slices in artificial cerebrospinal fluid (ACSF) at 35°C for 30-45 minutes. Afterwards, keep the slices at room temperature until recording.
- Place an acute slice on top of the MaxOne array and remove as much liquid as possible using pipettes. Use the Tissue Holder to slightly press onto and flatten the tissue onto the array. Make sure that the membrane is touching the tissue.
- Perfuse the acute slice with carbogen-loaded ACSF (31-33°C). Maintain perfusion of ACSF throughout the experiment.
- Perform HD-MEA recordings and stimulation experiments within 4-6 hours after dissection.
ACSF contents in mM: NaCl 125 , KCl 2.5, NaH₂PO₄ 1.25, MgSO₄ 1.9, Glucose 20, NaHCO₃ 25
What You Can Do
Capture Single Neuron and Network-Wide Field Potentials
Record high quality signals from active neurons on the MEA
MaxOne enables recording of neuronal activity across multiple scales at high spatio-temporal resolution.
- Both local field potentials and spikes from intact brain networks can be detected simultaneously.
- Low noise signals facilitate the extraction of neuronal activity features from experiments.
- Propagating field potentials across brain areas can be captured and analyzed.
Perform Large-Scale Mapping of Cells and Synaptic Projections
Extract and analyze the action potential spatial fields, axonal projections, and postsynaptic signals of every active neuron in the brain tissue. MaxOne can detect spiking neurons in brain slices and can elicit neuronal activity by electrical stimulation.
- A neuronal activity map can be extracted to identify areas of the brain slice with spiking neurons.
- Spiking frequency
- Postsynaptic events can be revealed by spike-trigerred averaging as a slow +/- signal post-spike. (M. Shein-Idelson, et al., Nat. Methods, 2017)
MaxOne Tissue Holder
MaxOne Tissue Holder flattens the brain slice on the MEA for stable and reprocible experiments.
The tissue holder keeps the tissue pressed and fixed on the MEA throughout the experiment, in the presence of solution perfusion.
Brain Slice Applications
Nature Methods, 14 (9), pp. 882–889, 2017, ISSN: 1548-7091. Frontiers in Neuroscience, 10 , pp. 1-16, 2016, ISSN: 1662453X. Biosensors and Bioelectronics, 24 (7), pp. 2191-2198, 2009, ISSN: 09565663.
Nature Methods, 14 (9), pp. 882–889, 2017, ISSN: 1548-7091.
Frontiers in Neuroscience, 10 , pp. 1-16, 2016, ISSN: 1662453X.
Biosensors and Bioelectronics, 24 (7), pp. 2191-2198, 2009, ISSN: 09565663.