Brain Slices

MaxOne for Brain Slice Studies  

MaxOne enables researchers to perform label-free analysis of intact brain networks in vitro:

  • Acute brain slices
  • Organotypic brain slice cultures and organoids
  • Ex-vivo brain preparation (e.g., eye-brain from turtles)

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Protocol

Sample Acute Brain Slice Preparation for Recording

  1. After dissection and slicing, incubate the brain slices in artificial cerebrospinal fluid (ACSF) at 35°C for 30-45 minutes. Afterwards, keep the slices at room temperature until recording.
  2. Place an acute slice on top of the MaxOne array and remove as much liquid as possible using pipettes. Use the Tissue Holder to slightly press onto and flatten the tissue onto the array. Make sure that the membrane is touching the tissue.
  3. Perfuse the acute slice with carbogen-loaded ACSF (31-33°C). Maintain perfusion of ACSF throughout the experiment.
  4. Perform HD-MEA recordings and stimulation experiments within 4-6 hours after dissection.

ACSF contents in mM: NaCl 125 , KCl 2.5, NaH₂PO₄  1.25, MgSO₄  1.9, Glucose 20, NaHCO₃  25

What You Can Do

Capture Single Neuron and Network-Wide Field Potentials

Record high quality signals from active neurons on the MEA

MaxOne enables recording of neuronal activity across multiple scales at high spatio-temporal resolution.

  • Both local field potentials and spikes from intact brain networks can be detected simultaneously.
  • Low noise signals facilitate the extraction of neuronal activity features from experiments.

  • Propagating field potentials across brain areas can be captured and analyzed.

Perform Large-Scale Mapping of Cells and Synaptic Projections

Extract and analyze the action potential spatial fields, axonal projections, and postsynaptic signals of every active neuron in the brain tissue. MaxOne can detect spiking neurons in brain slices and can elicit neuronal activity by electrical stimulation.

  • A neuronal activity map can be extracted to identify areas of the brain slice with spiking neurons.
  • Spiking frequency

  • Postsynaptic events can be revealed by spike-trigerred averaging as a slow +/- signal post-spike. (M. Shein-Idelson, et al., Nat. Methods, 2017)

MaxOne Tissue Holder

MaxOne Tissue Holder flattens the brain slice on the MEA for stable and reprocible experiments.
The tissue holder keeps the tissue pressed and fixed on the MEA throughout the experiment, in the presence of solution perfusion.

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Publications —

Brain Slice Applications

Large-scale mapping of cortical synaptic projections with extracellular electrode arrays

Shein-Idelson, Mark; Pammer, Lorenz; Hemberger, Mike; Laurent, Gilles

Large-scale mapping of cortical synaptic projections with extracellular electrode arrays Journal Article

Nature Methods, 14 (9), pp. 882–889, 2017, ISSN: 1548-7091.

Abstract | Links | BibTeX

Multiple single-unit long-term tracking on organotypic hippocampal slices using high-density microelectrode arrays

Gong, Wei; Sencar, Jure; Bakkum, Douglas J; Jäckel, David; Obien, Marie Engelene J; Radivojevic, Milos; Hierlemann, Andreas

Multiple single-unit long-term tracking on organotypic hippocampal slices using high-density microelectrode arrays Journal Article

Frontiers in Neuroscience, 10 , pp. 1-16, 2016, ISSN: 1662453X.

Abstract | Links | BibTeX

Microelectronic system for high-resolution mapping of extracellular electric fields applied to brain slices

Frey, Urs; Egert, Ulrich; Heer, Flavio; Hafizovic, Sadik; Hierlemann, Andreas

Microelectronic system for high-resolution mapping of extracellular electric fields applied to brain slices Journal Article

Biosensors and Bioelectronics, 24 (7), pp. 2191-2198, 2009, ISSN: 09565663.

Abstract | Links | BibTeX

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